Advances In Biochemical Engineering Biotechnology Plant by J.-J. Zhong

By J.-J. Zhong

The hot achievements in engineering reviews on plant cellphone cultures are reviewed, incorporated are the fuel focus results and bioprocess integration for the improved productiveness of plant secondary metabolites. The metabolic engineering of plant secondary metabolite pathways and recombinant protein creation from genetically changed plant cells are brought. Large-scale plant micropropagation through somatic embryogenesis and furry roots is mentioned for effective propagation of desease-free, genetically uniform and big quantities of crops in vitro in significant quantities. Characterization and alertness of furry plant roots endowed with photosynthetic capabilities can be lined during this targeted quantity.

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In headspace gas fed shake flasks, Tate and Payne also looked for an effect of CO2 concentration on cell growth and found none [51]. Kim et al. grew Thalictrum rugosum cells in an airlift fermenter and found that addition of CO2 to the air feed had no effect on cell growth but increased formation of berberine, the desired product [57]. A significant concern involves aerated systems, as one would expect to use in a large-scale process [58]. Preliminary studies typically involve growth in small shake flasks with limited gas exchange.

Annua was flushed with a gas mixture of known composition, then sealed and placed on a rotary shaker. The gas headspace was sampled at several hour intervals; the measured concentrations from all the tests were used to calculate the O2 consumption and CO2 production rates. 4 Effect of Headspace Composition on Growth and Production of Artemisinin Four types of stoppered culture tests were performed as follows: a b c d New culture from 21-day-old inoculum, flushed with air, shaken at 95 rpm Same as ‘a’ except flushed with high CO2 , high O2 gas mixture Same as ‘a’ except tests were started with 7- or 8-day-old cultures Same as ‘a’ except inoculum was from a 10-day-old culture The initial specific rates of O2 consumption and CO2 production are calculated and interpreted, and the effect of diminishing O2 concentration on its specific consumption rate is investigated.

Canadensis Production of Paclitaxel: Dependence on Dissolved Gas Concentrations Paclitaxel is a plant-derived drug used in the treatment of breast, ovarian and lung cancers; clinical trials are underway for treatment of other cancers. The extraction and purification of paclitaxel was initially from Pacific yew trees T. brevifolia and shrubs and trees of other Taxus species: T. baccata, T. cuspidata, T. sumatrana, T. chinensis, T. yunnanensis and T. hicksii. Because the evergreen Taxus brevifolia grows slowly (roughly a foot of height and a half inch of trunk diameter per decade), other techniques were considered to produce the compound without destroying T.

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